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About the project

​From Waste Cooking Oil to Succinic Acid

Waste cooking oils represent an ecological issue with wide negative effects when it is not stored and recycled properly. These oils are a type of biomass that can be valorized through biotechnological processes to obtain valuable organic compounds. The residual cooking oil is a rich source of free fatty acids that can be metabolized by oleophilic microorganisms and bioconverted into different organic acids. The 'SuccinYst' project aims to use the waste cooking oil for the biosynthesis of succinic acid. Succinic acid is an organic compound with wide applicability in various industries (e.g. foods, pharmaceutics, biodegradable materials development), which can be biosynthesized by oleophilic yeasts, such as Candida zeylanoides. Within the 'SuccinYst' project, we target the sustainable use of the waste cooking oil to reduce the massive pollution of the environment (especially of the drinking water supplies), by the bioconversion of this residual biomass into valuable organic compounds such as succinic acid.

Aim of SuccinYst

The proposed project’s approach is to use the waste cooking oil for the biosynthesis of succinic acid by using the lipophilic yeast strain of Candida zeylanoides within fermentation processes. In this project we aim to analyze the yeast strain adaptation mechanism to lipidic substrates like residual cooking oils, and their capacity to biosynthesize the targeted metabolite.

Expected results

Within the SuccinYst project we estimate to obtain a complex chemical characterization of residual edible oils of different origins, and also a characterization of the process of residual edible oil metabolism by the yeast Candida zeylanoides and succinic acid biosynthesis.

Achieved results

The outcomes from SuccinYst project brought:

- new insights considering the use of lipophilic microorganisms to bioconvert renewable  biomass into bioactive compounds

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- new perspectives of collecting and

using waste cooking oils

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- new perspective of obtaining,

purifying, and using succinic acid

in various applications

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- new collaborations with the economic sectors for developing new innovative food products incorporating succinic acid

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Funding

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This work is supported by a grant from the Romanian Ministry of Education and Research, CNCS  - UEFISCDI, grant no. PN-III-P1-1.1-PD-2019-0679.

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